Culture of soil bacteria - (Jul/16/2009 )
I am working on cultivation of some soil bacteria also, and I use serial dilutions when plating and I am getting from 100 to 350 colonies per sample. Also, I would like to recommend TG medium for plating, since I have tried to use many different media, and this one gave me best results. It is a little bit time consuming process to make it, but as I have already said, it works. This is the paper where you can find recipe: Mierob Ecol (1989) 17:181-192 MICROBIAL ECOLOGY C) Springer-Verlag New York Inc. 1989. Phenotypical Divergences between Populations of Soil Bacteria Isolated on Different Media.
Roald Sorheim, Vigdis Lid Torsvik, and Jostein Goksoyr.
Thanks for your advices, Pito and GeorgeWolff. I will try that out. Hopefully I can get the best result for my cultivation. Also thank you, Tanja. Thanks for sharing useful information with me. I really appreciate it. Have a nice day, friends!