DAPI staining - standard criteria (Jul/13/2009 )

Hello, everybody
I have just begin my experiment about apoptosis assay using DAPI staining. But, I have some problems about How to classify the character of normal cell and apoptotic cell. I confused about the character of nuclei condensation. So, I attached the three images of treatment groups
.
Last picture is a control group.
Thank you to you all in advance
Best regards

stain with anti active caspase III + dapi and you'll see the difference between the cells

yacon project on Jul 13 2009, 01:23 AM said:

You should see smaller, fragmented nuclei (apoptotic bodies) as compared to control. Best thing is to follow the previous response.

Dominic on Jul 13 2009, 10:51 PM said:

Thank you so much for your answer. you mean only dapi it's not suitable for apoptosis assay right. But,why I have read many paper using this method?

the only thing i trust a nuclear stain to do is show me where the nucleus is

if you see more than 20 cells they start to blend into one - you need a clear marker - not a vague hint in the nucleus

maybe there are people out there using the nuclear method - real question is why trust their work when its obviously so subjective

dom