CFSE staining protocol - (Jun/27/2009 )
What kind of results did you get?
miBunny on Aug 11 2009, 03:50 AM said:
I think that I didn't stain the cells, because I couldn't find any fluorescence in FL1. What I tried it was to add 10 ml of 5 uM CFSE to a petri dish with PC-3 cells attached, leave it at 37 degrees and then replaced it with RPMI 10% FCS, leave 30 min and the replace with other RPMI 10% and leave for 3 days
I think that I didn't stain the cells, because I couldn't find any fluorescence in FL1. What I tried it was to add 10 ml of 5 uM CFSE to a petri dish with PC-3 cells attached, leave it at 37 degrees and then replaced it with RPMI 10% FCS, leave 30 min and the replace with other RPMI 10% and leave for 3 days