Differentiating Thp-1 into macrophage - (Jun/24/2009 )
I also would like to know if it's possible to store the medium for let's say, 1 month. Also, do these cells keep proliferating after differentiated?
I've read a lot of papers about PMA and finally I decided to do fresh medium every time I need to. Everything works well:)
Macrophages are mature form of monocytes and they don't proliferate after differentiated.