Sheep Ab - suitable conjugate (Jun/18/2009 )

Dear all,

I have sheep antibody and I don't know the suitable conjugate, do you think Alexafluor 488 goat antirabbit is Ok? any suggestions would be appriciated.

Why would an anti-rabbit Ab work against a sheep primary? You need some anti-sheep secondary...

rimal on Jun 19 2009, 02:07 AM said:

For sheep antibodies, anti-goat immunoglobulin (eg rabbit anti-goat IgG) usually work very well. You probably have a suitable reagent hanging around in your lab.

Hope this helps

klinmed on Jun 20 2009, 03:34 AM said:

this my first time using antibodies, I have donkey anti sheep is that OK?

rimal on Jun 20 2009, 05:11 PM said:

Should be fine.

Sheep and goat immunoglobulins show a high degree of homology, so an anti-goat or anti-sheep conjugate should work.
Companies that produce primary antibodies in goats or sheep normally supply suitable secondaries.