x-ray films vs direct imaging - Preferred method for western development (Jun/18/2009 )

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so what is the problem in that.....you can go through different sites and books and enhance your knowledge in this field...

-lola-

Thanks for the sharing the additional information.

seo company

-ravirajcdc-

And moving to camera is interesting because films boxes are expensive!

-nicolas-

LostintheLab on Jun 18 2009, 06:04 PM said:

I"ve used both X-rays and digital imaging- we have a Fujifilm CCD system in the lab now, which is very convient- less mess and less time for developing as our nearest dark room in 2 floors below us and we have to book in. The advantage of the digital images is that they are easily transferred and you can carry out some quantitative analysis on them more easily too.
I agree the sensitivity of the films can sometimes be better, but its not been an issue for me. I'm pretty converted to the Digital imaging now.
We use in conjunction with ECL, but our system allows us to take images in a number of wavelengths so can be used for other methods too.

Hii,

I'm new in this field and had same doubt in my mind. Thanks for Clarification.
Keep Posting. I would like to know more about New Techniques in Imaging.

SEO Services India

-SEO Services India-

Hello,

Very nice forum for discussion.Thanks for sharing with us.

Regards
Website Developers India

-Website Developers India-

The benefit of the digital pictures is that they are effortlessly moved and you can convey out some quantitative investigation on them more effortlessly too. I acquiesce the sensitivity of the movies can occasionally be better, but its not been an topic for me. I'm attractive altered to the Digital imaging now.

-shane-

I never develop X-ray film. Could you give me a protocol or same advice?

-alinud-

Modern camera-based systems are to prefer since developing immunoblots have a longer linear range of detection; besides, classical films need a threshold level of light for darkening; a third advantage is that normally camera-based systems are offered with a professional gel documentation and analysis software

almost a doctor on Thu Jun 18 08:54:16 2009 said:

Hi guys,
I've been asked to source all the equipment and reagents needed to set up western blot in the lab, starting from scratch (all we have is a Nupage electrophoresis tank). I know my preferences for transfer system, membranes, reagents.... but I'm having doubts on the developing method. In the past I've always used ECL and x-ray films, but I know more and more labs these days favour other chemiluminescent reagents and imaging as you get a better control on exposure and a better dynamic range.

So here's my question.

What method do you use to develop a western? :lol:

Please share your opinions

Thanks! :lol:

-Inmost sun-

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