Visualizing Small Peptides - Using SDS-PAGE to check for expression of small proteins (Jun/15/2009 )
Fredriksen on Jun 17 2009, 03:39 PM said:
I suppose a longer development time just doesn't cut it? I'm using a silver stain kit from GE.
no. longer development time just makes the background darker. restaining appears to enhance silver binding.
we use a homemade (labmade?) modification of merril's method (if you want, i'll give you the reference when i get my book back, i was supposed to have it yesterday).
-mdfenko-
mdfenko on Jun 17 2009, 01:00 PM said:
Fredriksen on Jun 17 2009, 03:39 PM said:
I suppose a longer development time just doesn't cut it? I'm using a silver stain kit from GE.
no. longer development time just makes the background darker. restaining appears to enhance silver binding.
we use a homemade (labmade?) modification of merril's method (if you want, i'll give you the reference when i get my book back, i was supposed to have it yesterday).
That would be awesome if you could do that for me
-Fredriksen-
as soon as i get my book back, then, i'll give you the reference and our formulation with modifications.
-mdfenko-
mdfenko on Jun 18 2009, 09:39 AM said:
as soon as i get my book back, then, i'll give you the reference and our formulation with modifications.
Had you ever used any of the SYPRO staining methods for the small peptides as well? I tried double silver staining last week and I still could not see below 10kD. I guess I either need your formulation or I need to try something else.
-Fredriksen-
i'm sorry that i haven't posted yet. i am still waiting for the return of my methods book (and getting really p.o.ed that my requests have been ignored). i hope to be able to post soon.
-mdfenko-
i finally got my methods book back.
here is the protocol as modified by my lab, it is in rich text format.
sorry it took so long.
-mdfenko-
Thanks! I'll try this soon!
-Fredriksen-