In situ - (Jun/07/2009 )
I am doing in situ hybridization with zebrafish embryos. The protocol calls for rinsing in MeOH and storing the fixed embryos in MeOH overnight at 4C. I forgot to do the "storing" part although I did the rinsing portion and went right ahead to hydrating and the procedure.
What do I do? Is it ok if I skipped the "storing" part? How will it affect my results? Why does one use MeOH?
is the methanol your sole fixative?
if yes - then your buggered - your tissue wont be fixed and any future work done to it worthless
btw - you only need to ask the question once - three times is just silly