Label with 35S -met - (Jun/06/2009 )
Hi everyone
As you know we can label the protein of interest with 35S-Met only if it contains a few met residues.
My proteins have 7/(425 aa), 5 /( 250 aa), 8/(330 aa) and 5/( 185 aa) met residues respectively,
Are they enough to detect easily by autoradiography?
Is there any rule for these?
Thank you so much in advance.
-saad-
You should be able to detect them, usually 3-5 is enough.
-bob1-