transient expression - best time to detect the mRNA? (Jun/01/2009 )
Doing transient expression transfecting a plasmid in HEK293:
What is the best time after transfection to do RNA extraction and RT-PCR to detect the mRNA of the gen in the plasmid?
And what is the minimum time?? Do you think after 24 h I could detect easily the mRNA???
Thanks in advantage!!!
I would say the best time frame is around 48 hrs. The minimum time is less than 24 hrs. This can be told by transfecting a GFP plasmid.