colonies on a plate - how long will they last? (May/21/2009 )
I streaked out some colonies on some LB+ Amp plates back in December 2008 for DNA sequencing. I picked out the colonies for my experiment and then stored the plates in the 4*C since then. I want to repeat the experiment again now using these plates.
Is this a good way for long term storage of these plates?
The cells on those plates are almost certainly dead. For long term storage, grow cultures of your cells in LB or another rich medium, add sterile 80% glycerol solution to give a final 15% glycerol concentration in your medium, and freeze at -80C for storage indefinitely. If you are desperate to recover a plasmid from your (now dead) cells on the plate, it might be possible to prep DNA and transform living cells from your colonies, but I would not count on it. Storing plasmid DNA is also an effective means of long term storage.
phage434 on May 21 2009, 06:55 AM said:
yeah, i agree with that. i myself have tried to revive colonies from a month old plate, it did not work....i wouldn't rely on such an old plate.