Antibody to mouse antigen raised in mice? How it is working? - (May/19/2009 )
Thanks a lot!!!!!
little mouse on May 20 2009, 03:41 PM said:
katenkak on May 20 2009, 02:38 PM said:
Thank you for the suggestion. Do you know very economical procedure for this? I buy commercial antibody, so there are very little and of course expensive-nothing to waste.
little mouse on May 20 2009, 01:15 PM said:
you could also directly label the primary antibody (biotin or Alexa-488)
no economical procedure. It's doubling the price of your antibody if not worse. That's why it would be better to buy it already labeled if possible.
I used reactive biotin from Pierce to label surface proteins (EZ-link sulfo NHs-LC biotin) that worked fine, and for fluorescent labeling, I used a kit from molecular probes to label monoclonal antibodies. But it is expensive (we were producing our own monoclonal antibodies, unless I would have bought them already labeled).
I don't remember exactly the price.
but If you already have the antibody, have a try with and without the primary antibody to see if you really have background. If you are working on tissues, I know there is a treatment to get rid of the Ig inside the tissue, but I don't know how.