Help cloning!!! - As usual cloning is not working! (May/08/2009 )
I would prep and at least RE cut four to see what bands were present. If you have cheap easy sequencing, that would be best. You're working too hard if you are analyzing 150 colonies (at least without robotics to help).
phage434 on May 13 2009, 04:19 AM said:
I analyzed three clones and if they look bigger than the original plasmid I analyzed them by fast digestion with BamHI and XhoI, so I can see only the linear vector, I can't see the insert, so I do think there is something smaller that enter the plasmid or double digestion is not working!!