H2O2, how freshl should it be? - (Apr/16/2009 )
Hi guys!
I am new to immunohistochemistry field and got a suspicion that 0.3% H2O2 used to block endogenous peroxidase should be freshly diluted from stock, since my slides turned brown almost immediately after I added developing solution (mine H2O2 was prepared some weeks ago and was stored in refrigirator). Is that right? Should I prepare H2O2 right before I use it?
Thank you for answering!
katenkak on Apr 16 2009, 04:56 AM said:
I am new to immunohistochemistry field and got a suspicion that 0.3% H2O2 used to block endogenous peroxidase should be freshly diluted from stock, since my slides turned brown almost immediately after I added developing solution (mine H2O2 was prepared some weeks ago and was stored in refrigirator). Is that right? Should I prepare H2O2 right before I use it?
Thank you for answering!
If the peroxide was in a clear bottle, it was definitely bad. Hydrogen peroxide is light sensitive. I don't see why you shouldn't be able to store it for a while, but make sure you protect it from light. If you don't have any opaque bottles, wrap one in aluminum foil. Also, I tend to prefer to use freshly diluted solutions, though I can't say whether it necesarily matters.
Thank you,
I had my diluted H2O2 in 50 ml plastic tube, so it was definetely exposed to light, but strangely enough the stock solution is not in a dark bottle (white opaque). I'll try freshly diluted peroxide as you do.
Best
If the peroxide was in a clear bottle, it was definitely bad. Hydrogen peroxide is light sensitive. I don't see why you shouldn't be able to store it for a while, but make sure you protect it from light. If you don't have any opaque bottles, wrap one in aluminum foil. Also, I tend to prefer to use freshly diluted solutions, though I can't say whether it necesarily matters.