Newborn Bovine Serum - Is this false economy? (Mar/30/2009 )
Does anyone have experience using Newborn Bovine Serum instead of FBS?
It is only a fraction of the cost of FBS so I was thinking of trying it for some of my more robust cells/assays.
FCS is the only one of the three that does not contain antibodies.
ECM factors, such as fibronectin, are also present in serum.
-Heat treatment inactivates complement and should always be done with FCS!
These cytokines are not present in BSA.
Many thanks. I had come across papers which used either serum or a mix of serum sources but very little on the rational for the particular choice and what changes this makes to assay outcomes.
Anyway, it may all be a mute point because after I posted the question I realised that a major change in media will cause too much managerial asperity and it would be best to maintain continuity with what has gone before.