positive control for BSP? - (Mar/24/2009 )
51 degree is too low. I have never used such low Ta. try 55C.
-pcrman-
but my primers Tms are 53 and 54 (according to Primer 3), its 47 according to IDT, should i give a try with 55?
pcrman on Mar 25 2009, 07:16 AM said:
51 degree is too low. I have never used such low Ta. try 55C.
-epigenetics-
epigenetics on Mar 25 2009, 04:23 PM said:
but my primers Tms are 53 and 54 (according to Primer 3), its 47 according to IDT, should i give a try with 55?
Yes!
I even use 2-3°C more.
-battlebee-