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positive control for BSP? - (Mar/24/2009 )

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51 degree is too low. I have never used such low Ta. try 55C.

-pcrman-

but my primers Tms are 53 and 54 (according to Primer 3), its 47 according to IDT, should i give a try with 55?


pcrman on Mar 25 2009, 07:16 AM said:

51 degree is too low. I have never used such low Ta. try 55C.

-epigenetics-

epigenetics on Mar 25 2009, 04:23 PM said:

but my primers Tms are 53 and 54 (according to Primer 3), its 47 according to IDT, should i give a try with 55?


Yes!
I even use 2-3C more.

-battlebee-
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