How many bp should be left at 5' and 3' flanking regions needed for miRN - miRNA (Mar/23/2009 )
I am anxious to know.
How many bp should be left at 5' and 3' flanking regions to constucting a miRNA vector?
Some people left 600bp, some people left 100 or 200 bp. How many should be enogh for expressing miRNA?
Is there any article about this?
Thank you pcrman.
If a longer bp was remained, any problem will happen?
pcrman on Mar 24 2009, 07:01 AM said:
I tried 30 and 60.....30 was more than enough!!
but maybe i was just lucky.
I have cloned and generated over 600 human lentiviral miRNA collection. There are 100bp flanking sequences on both sides of pre-miRNA and they work great.