Transfection contamination? - (Mar/23/2009 )
Hi, I know that culture medium could be of influence to show a contamination! You probably use antibiotics or fungizone (amphotericin in one and maybe not in the other?
Also yeast prefers a certain media probably as some of the cancer cells I transfect die in DMEM and grow rapidly in RPMI!
Good luck! Hope your cells have survived in your last experiment!
Thank you guys! I've found out the problem is the siRNA oligo. It have been contaminated. So I just discard it and buy a new one. Hopw things will be well soon.