Transfection contamination? - (Mar/23/2009 )
Hi, I know that culture medium could be of influence to show a contamination! You probably use antibiotics or fungizone (amphotericin 
in one and maybe not in the other?
Also yeast prefers a certain media probably as some of the cancer cells I transfect die in DMEM and grow rapidly in RPMI!
Good luck! Hope your cells have survived in your last experiment!
-memo-
Thank you guys! I've found out the problem is the siRNA oligo. It have been contaminated. So I just discard it and buy a new one. Hopw things will be well soon.
-proteinrunner-
Sorry to hear that but at least its sorted now.
Good luck in the future
Cotchy.
-cotchy-