Limitations of Animal Tissue Cultures - (Mar/16/2009 )

I have a question guys?

I am doing sputum processing and when I finhsed from the haemocytometer count, i have to centrifuge the the filtrate of the sample and then remove the supernatant and leaving the cell pellet, I need to know how THEN resuspend the cell pellet in PBS and then adjust the cells/volume to give me 0.5 million for preparation of cytospin slides!

Thank you

i need your help please , tell me the method
how i make study about new chemotheraputic agent effect on cells of paranasal sinuses and fungal cells
please help quickly
thank you in advance