Help with Real Time PCR Well To Well Variation - (Mar/15/2009 )
dtae on Wed Aug 18 23:08:18 2010 said:
not sure if people are still paying attention to this thread: but any further thoughts on the necessity of spinning down plates before putting in the thermocycler. A post-doc in my lab has reasoned that it doesn't really matter with her hotstart assay because the heat will disrupt the bubbles and cause the solution to evaporate and then condense regardless. Is this faulty reasoning?
She has a point, but it's good practice nonetheless.
e: It's not like we can ever see it while it's happening sadly.