How to maintain cells at a specific apoptosis stage - (Mar/07/2009 )
With DAPI and fluorescence microscopy you can fix beforehand and then permeabilise the cells with a 10 min incubation in PBS or TBS containing 0.1% Tween 20 or Triton X-100 to allow the dye to enter the cells.
NemomeN on Mar 9 2009, 10:11 AM said:
If I were you, I would try this method. But I am concerned about dying out of fluorecence during the treatment. Since in some flow cytometry analysis, intracellular antibody were used (Oct4 as I know). And I remeber the cells need to be fixed. And you could count on some related litterature to optimal your condtion. May it help you and good luck.
DAPI will work, but you will need to permeablize after you fix so that it can enter. If you fix before you stain, your annexin signal will be all false positive