artifacts in inmunofluorescence - (Mar/03/2009 )

Hi all,
Does anybody know if methanol or PF and methanol introduces artifacts of some kind in inmunofluorescence?

Paraformaldehyde (PFA) can cause artifacts through autoflourescence of undissolved PFA crystals.

sela on Mar 3 2009, 03:48 PM said:

There are papers showing that certain fixations can influence the localization and staining of a specific protein. Check out this paper: PMID: 1527176