chloramphenicol mini-prep - (Feb/25/2009 )
Can chloramphenicol be added into O/N culture containing plasimd with R6K replicon? If yes, how much of it should I add and how long is the incubation time? If no, is there anyway to increase the plasmid yield?
Don't know if this would help but I use Ch for E.coli cells transformed with BAC clones. I use 12.5 ug/ml and incubate for 16 hours. To increase plasmiud yield, I inoculate 6 tubes with 3 mL media pool the yield.
Nrelo on Feb 26 2009, 08:31 AM said: