Dicer-KO - are lines available commercially (Feb/12/2009 )
I was just wondering if the Dicer Knockout cell lines/conditional KO mice were available commercially.. I know its been a while that people have been using them for, I just didn't know if you could get them somewhere!
it_never_ends on Feb 12 2009, 02:50 PM said:
The easiest way is to find collaborators who are using them
otherwise, you can also ask for generation of a gene trapped Dicer knockout from TIGM, either get the ES cells and make your own mice or have them do it for you, at about $15k, takes more than 1 yr
otherwise, if you want target knockout, there are other companies to make it for you, takes longer and more expensive
The faster/cheaper approach is a Dicer knockdown.
Wienholds E, Koudijs MJ, Van Eeden FJ, Cuppen E, Plasterk RH. The microRNA-producing enzyme Dicer1 is essential for zebrafish development. Nat Genet. 2003 Nov;35(3):217-8. Epub 2003 Oct 05.
Place RF, Li LC, Pookot D, Noonan EJ, Dahiya R. MicroRNA-373 induces expression of genes with complementary promoter sequences. Proc Natl Acad Sci U S A. 2008 Feb 5;105(5):1608-13. Epub 2008 Jan 28.
it_never_ends on Feb 12 2009, 01:50 PM said:
Looks like Dicer CKO mice are available from Jax, two strains.