Electroporation - does anybody have an up to date protocol (Feb/12/2009 )

hi all,

i know i'm being kind of vague but does anyone have a up to date/current protocol for using a biorad (not sure of the make but its old!!) or indeed any other electroporation system if they are all similar

We are using it to insert a plasmid into our cells, as we have had no uptake using lipofectamine.

Any advice would be great.

cotchy on Feb 12 2009, 07:39 AM said:

Electroporation of eukaryotic cells is an efficient but tricky business. Some cells survive the process well, some just don't and die in droves.

You will need to optimize the electroporation protocol for your particular cells, and for the electroporator you have.

Perhaps in this link you will find electroporation protocol for your cells.

Amaxa nucleofection is well standardized for many different cells, particulary for difficult to transfect cells and primary cells.

Bio-rad had some info on the protocol (setting) for electroporation. We used to have an old biorad electroporator and we used the settings on the site. It worked quite well.

cellcounter on Feb 12 2009, 10:45 AM said:

I don't use it, but from the results I've seen Amaxa is the way to go these days.

thanks all, i had a look on the biorad site and found some good protocols

cheers,