preparation of SSC - (Feb/11/2009 )

Hi,

I want to prepare SSC(standard sodium citrate) can anyone help me with the froumula.

-bluebird-

bluebird on Feb 11 2009, 09:37 AM said:

Hi,

I want to prepare SSC(standard sodium citrate) can anyone help me with the froumula.

Have you check in Maniatis, the lab manual for molecular biology.

-scolix-

Try this one from this site: http://openwetware.org/wiki/Oneill_Lab:Chemicals#20x_SSC

20x SSC

Recipe to make 4L

* Dissolve 701.28g NaCl and 352.92g NaCitrate
* pH to 7.0
* Bring final volume to 4L
* Autoclave if necessary

-scolix-

Many thanks for help :blink:

-bluebird-