Centrifuging to separate bacteria from molecules - (Feb/03/2009 )
gebirgsziege on Feb 6 2009, 05:43 PM said:
Maybe you should look for some kind of centrifugation-technique with a selective barrier....
but have you ever thought about this: bac A is producing the substance that inhibits bac B only if directly challenged by it?? So some trigger substance of e.g. Bac Bs membrane is needed to let bac A produce the substance?
but have you ever thought about this: bac A is producing the substance that inhibits bac B only if directly challenged by it?? So some trigger substance of e.g. Bac Bs membrane is needed to let bac A produce the substance?
Yes, I`ve thought of that, however bac A also inhibits other bacteria of different species which suggests its not a species specific trigger.
-Tom-
Tom on Feb 6 2009, 12:24 PM said:
If I separate the two by a 0.2 semipermeable membrane which prevents the bacteria from interacting, but allows the medium and therefore their secreted substances to pass, there is very less inhibition. I've a larger pore size (0.45) of a different material and found even less inhibition.
Does this make sense? Why would using a larger pore size (= more stuff through) result in a decrease in inhibition ("larger pore size" = "even less inhibition") if the cause of the inhibition is a secreted substance?
-HomeBrew-
agree homebrew, and i doubt the concept has legs. Wonder what data validates presumption that it allows free movement.
-GeorgeWolff-
HomeBrew on Feb 7 2009, 01:13 PM said:
Tom on Feb 6 2009, 12:24 PM said:
If I separate the two by a 0.2 semipermeable membrane which prevents the bacteria from interacting, but allows the medium and therefore their secreted substances to pass, there is very less inhibition. I've a larger pore size (0.45) of a different material and found even less inhibition.
Does this make sense? Why would using a larger pore size (= more stuff through) result in a decrease in inhibition ("larger pore size" = "even less inhibition") if the cause of the inhibition is a secreted substance?
one instance where this can happen if there was the presence of a immune factor of a larger size that would pass through 0.45 but not 0.2. Thus filtering through a 0.2 stops the immune factor from passing but allows the inhibitory factor to pass. This is seen in several bacteria such as in the case of lantibiotics.
O.K , all i asked for was if anyone could help with the centrifugal speed in separating bacteria from molecules. Now its turning into an open defense of my thesis
If you cant help with the original question lets just be say we dont know and leave it at that eh?! -Tom-
Your call, Tom. But you might consider the "defense" a favor. These are questions that will be asked and it's easier to hear them now and answer them while your doing the research than at the end.
good luck
-GeorgeWolff-