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PVDF vs. Nitrocellulose for Western blot - (Jan/31/2009 )

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WblotMaster on Jun 3 2010, 07:41 PM said:

2. SDS inhibits protein transfer to nitrocellulose membranes. Can aid transfer of large proteins to PVDF

sds will aid in the transfer of large proteins onto nitrocellulose, too. it will also interfere with binding to pvdf (one effect is that it makes the protein travel too fast to be captured). methanol in the buffer strips it off the protein so that it can bind to either membrane.

you can get this and more information from the millipore protein blotting handbook.

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