Important literature for real time PCR users - Important documents for beginners as well as advanced users (Jan/29/2009 )
Anyone have the next paper?
Bonefeld BE, Elfving B, Wegener G: Reference genes for normalization: a study of rat brain tissue.
Synapse 2008, 62:302-309.
check your pm.
Like MIAME for microarray paper, MIQE is a useful guideline for those who want to do qPCR.
Here is the website: http://www.rdml.org/miqe.php
very useful and thx a lot
The following article I found especially interesting concerning use of reference genes for normalization. Please note that I have already highlighted this article - sorry if that is bothersome!
Thanks for all the other ones you posted!
This is great tea-test thank-you so much! I have just started molecular biology and qPCR so this is amazing!
Thanks also Sanjiun and flammaefata - such a great help!
It should be very usefull, thanks a lot!
Thank you very much for these compiled protocols. I would like to read something about HRM curve analysis using SYBR green on an Applied Biosystem 7500 RT-PCR. Actually that is the PCR in my Lab and I have to do HRM with it.
Any help regarding this would be greatly appreciated.
Thank you in advance.
thank you for these papers！