Protocol Online logo
Top : New Forum Archives (2009-): : Molecular Cloning

kpi ? - (Jan/29/2009 )

Hi, i must prepare 50 mM Kpi pH 7,4. How? what I must weigth and how to reach the rigth pH?
Thanks

-FRAROAR-

FRAROAR on Jan 29 2009, 05:06 AM said:

Hi, i must prepare 50 mM Kpi pH 7,4. How? what I must weigth and how to reach the rigth pH?
Thanks


hi,
You may prepare 500mM (10x) KH2PO4 or K2HPO4, pH to 7.4 with KOH.

-WHR-