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Amyloid beta labelling - (Jan/27/2009 )

Hell all,
I have a question, do you use some special labels for amyloid beta peptide? I have to make sure that the conformation of the specific oligomer should not be affected. I got to see them in live cells.. Any suggestions?

-BioSaint-

BioSaint on Jan 27 2009, 05:51 PM said:

Hell all,
I have a question, do you use some special labels for amyloid beta peptide? I have to make sure that the conformation of the specific oligomer should not be affected. I got to see them in live cells.. Any suggestions?


That might be difficult. What exactly do you want to do? Study the movement of A-beta or simple localization?

-scolix-


That might be difficult. What exactly do you want to do? Study the movement of A-beta or simple localization?


*** Starting with localization.. FITC is ok.. But any other stuff?

-BioSaint-

Have you thought about using GFP fused to A-beta? They may not work but they could also work.

-scolix-

Yeah, thought about it mate, but it may harm the aggregation pattern..

-BioSaint-