Methprimers - (Jul/22/2021 )

Hi,

I designed primers using methprimer.

1. Before using methprimer. I BLAST my promoter sequence to confirm its from my gene of interest, and it was

2. I than put he promoter sequence on methprimer and it generated primers

3. When I blasted the primers (methylated primers and unmethylated primers), the results dont match my gene of interest.

Is there anything Im doing wrong? I thought the methylated primer sequence would match my gene using BLAST

Thank you

I don't know.