# Papain activity calculation - (Apr/20/2018 )

Hi,

I am confused as how to calculate the activity of Papain.

It's in lyophilized form and I want to prepare 200 µl. So, how can I calculate the activity.

I have the following information: Size - 1 u/vial and Act: 26.1 u/mgP; 57% protein

What should I dissolve it in to prepare the solution?

Thanks

Do you have an activity that you want? Or do you want activity per ul?

If the latter, then it is simple 1 U/200 ul = 0.005 U/ul

-bob1-

bob1 on Fri Apr 20 21:14:04 2018 said:

Do you have an activity that you want? Or do you want activity per ul?

If the latter, then it is simple 1 U/200 ul = 0.005 U/ul

Hi Bob,

I want an activity of 20 units/ml. How do I calculate that?

200 ul is 0.2 of a ml.

For this you can use the n=c*v

n= 20 u/ml * 0.2 ml

n=4 U

-bob1-

bob1 on Sat Apr 21 03:42:54 2018 said:

200 ul is 0.2 of a ml.

For this you can use the n=c*v

n= 20 u/ml * 0.2 ml

n=4 U

So, how much of the solution (example, PBS) should I add to the lyophilized powder to make it 4U? Also, is PBS is the best solution to dissolve the Papain or do you recommend dissolving it in any other solution?