Serial dilution - (Nov/19/2015 )
Hello,
I am confused on how to do a serial dilution.
It says:
Dilute standard from 1ng/ml to zero in diluent.
And I want to have 5 or 6 points:
1. 1ng/mL
2. 0.5ng/ml,
3. 0.25 ng/ml,
4. 0.125 ng/ml (125pg/ml),
5. 62.5 pg/ml,
6. 31.25 pg/ml
Kindly advice how can I serially dilute this.
Mad Researcher on Thu Nov 19 07:42:33 2015 said:
Hello,
I am confused on how to do a serial dilution.
It says:
Dilute standard from 1ng/ml to zero in diluent.
And I want to have 5 or 6 points:
1. 1ng/mL
2. 0.5ng/ml,
3. 0.25 ng/ml,
4. 0.125 ng/ml (125pg/ml),
5. 62.5 pg/ml,
6. 31.25 pg/ml
Kindly advice how can I serially dilute this.
you want to go from 1ng/ml to 31,25pg/ml ?
take part of the 1ng/ml and add an equal volume of diluent to make 0.5ng/ml.
take part of the 0.5ng/ml and add an equal volume of diluent to make 0.25ng/ml.
take part of the 0.25ng/ml and add an equal volume of diluent to make 0.125ng/ml.
etc.
pito on Thu Nov 19 11:25:47 2015 said:
Mad Researcher on Thu Nov 19 07:42:33 2015 said:
Hello,
I am confused on how to do a serial dilution.
It says:
Dilute standard from 1ng/ml to zero in diluent.
And I want to have 5 or 6 points:
1. 1ng/mL
2. 0.5ng/ml,
3. 0.25 ng/ml,
4. 0.125 ng/ml (125pg/ml),
5. 62.5 pg/ml,
6. 31.25 pg/ml
Kindly advice how can I serially dilute this.
you want to go from 1ng/ml to 31,25pg/ml ?
Yes...
mdfenko on Thu Nov 19 14:07:30 2015 said:
take part of the 1ng/ml and add an equal volume of diluent to make 0.5ng/ml.
take part of the 0.5ng/ml and add an equal volume of diluent to make 0.25ng/ml.
take part of the 0.25ng/ml and add an equal volume of diluent to make 0.125ng/ml.
etc.
So, 1 ng/ml is my stock and if it comes in 1ml then:
I take 0.5 ml of my stock + 0.5 of H2O -- 1
0.5 ml of 1 + 0.5 ml of H2O -- 2
0.5 ml of 2 + 0.5 ml of H20 -- 3
and so on
Am i correct?
Mad Researcher on Thu Nov 19 14:15:52 2015 said:
mdfenko on Thu Nov 19 14:07:30 2015 said:
take part of the 1ng/ml and add an equal volume of diluent to make 0.5ng/ml.
take part of the 0.5ng/ml and add an equal volume of diluent to make 0.25ng/ml.
take part of the 0.25ng/ml and add an equal volume of diluent to make 0.125ng/ml.
etc.
So, 1 ng/ml is my stock and if it comes in 1ml then:
I take 0.5 ml of my stock + 0.5 of H2O -- 10.5 ml of 1 + 0.5 ml of H2O -- 2
0.5 ml of 2 + 0.5 ml of H20 -- 3
and so on
Am i correct?
if your stock is 1ng/ml and you then take 0,5ml of this and add 0,5ml water you will have in total 0.5ng (since you took 0.5ml from a 1ng/1ml stock) of the stock in 1ml, thus 0.5ng/ml.
And you can keep doing this:
take 0,5ml from the "new" stock , add 0.5ml water... you keep diluting it 1/2 each step.
But this is pretty basic, you should understand this, know how this works.
Mad Researcher on Thu Nov 19 14:15:52 2015 said:
mdfenko on Thu Nov 19 14:07:30 2015 said:
take part of the 1ng/ml and add an equal volume of diluent to make 0.5ng/ml.
take part of the 0.5ng/ml and add an equal volume of diluent to make 0.25ng/ml.
take part of the 0.25ng/ml and add an equal volume of diluent to make 0.125ng/ml.
etc.
So, 1 ng/ml is my stock and if it comes in 1ml then:
I take 0.5 ml of my stock + 0.5 of H2O -- 10.5 ml of 1 + 0.5 ml of H2O -- 2
0.5 ml of 2 + 0.5 ml of H20 -- 3
and so on
Am i correct?
if 1, 2 and 3 are 0.5ng/ml, 0.25ng/ml and 0.125ng/ml, respectively, then yes.