# Serial dilution - (Nov/19/2015 )

Hello,

I am confused on how to do a serial dilution.

It says:

Dilute standard from 1ng/ml to zero in diluent.

And I want to have 5 or 6 points:

1. 1ng/mL

2. 0.5ng/ml,

3. 0.25 ng/ml,

4. 0.125 ng/ml (125pg/ml),

5. 62.5 pg/ml,

6. 31.25 pg/ml

Kindly advice how can I serially dilute this.

Mad Researcher on Thu Nov 19 07:42:33 2015 said:

Hello,

I am confused on how to do a serial dilution.

It says:

Dilute standard from 1ng/ml to zero in diluent.

And I want to have 5 or 6 points:

1. 1ng/mL

2. 0.5ng/ml,

3. 0.25 ng/ml,

4. 0.125 ng/ml (125pg/ml),

5. 62.5 pg/ml,

6. 31.25 pg/ml

Kindly advice how can I serially dilute this.

you want to go from 1ng/ml to 31,25pg/ml ?

-pito-

take part of the 1ng/ml and add an equal volume of diluent to make 0.5ng/ml.

take part of the 0.5ng/ml and add an equal volume of diluent to make 0.25ng/ml.

take part of the 0.25ng/ml and add an equal volume of diluent to make 0.125ng/ml.

etc.

-mdfenko-

pito on Thu Nov 19 11:25:47 2015 said:

Mad Researcher on Thu Nov 19 07:42:33 2015 said:

Hello,

I am confused on how to do a serial dilution.

It says:

Dilute standard from 1ng/ml to zero in diluent.

And I want to have 5 or 6 points:

1. 1ng/mL

2. 0.5ng/ml,

3. 0.25 ng/ml,

4. 0.125 ng/ml (125pg/ml),

5. 62.5 pg/ml,

6. 31.25 pg/ml

Kindly advice how can I serially dilute this.

you want to go from 1ng/ml to 31,25pg/ml ?

Yes...

mdfenko on Thu Nov 19 14:07:30 2015 said:

take part of the 1ng/ml and add an equal volume of diluent to make 0.5ng/ml.

take part of the 0.5ng/ml and add an equal volume of diluent to make 0.25ng/ml.

take part of the 0.25ng/ml and add an equal volume of diluent to make 0.125ng/ml.

etc.

So, 1 ng/ml is my stock and if it comes in 1ml then:
I take 0.5 ml of my stock + 0.5 of H2O -- 1

0.5 ml of 1 + 0.5 ml of H2O -- 2

0.5 ml of 2 + 0.5 ml of H20 -- 3

and so on

Am i correct?

Mad Researcher on Thu Nov 19 14:15:52 2015 said:

mdfenko on Thu Nov 19 14:07:30 2015 said:

take part of the 1ng/ml and add an equal volume of diluent to make 0.5ng/ml.

take part of the 0.5ng/ml and add an equal volume of diluent to make 0.25ng/ml.

take part of the 0.25ng/ml and add an equal volume of diluent to make 0.125ng/ml.

etc.

So, 1 ng/ml is my stock and if it comes in 1ml then:
I take 0.5 ml of my stock + 0.5 of H2O -- 1

0.5 ml of 1 + 0.5 ml of H2O -- 2

0.5 ml of 2 + 0.5 ml of H20 -- 3

and so on

Am i correct?

if your stock is 1ng/ml and you then take 0,5ml of this and add 0,5ml water you will have in total 0.5ng (since you took 0.5ml from a 1ng/1ml stock) of the stock in 1ml, thus 0.5ng/ml.

And you can keep doing this:

take 0,5ml from the "new" stock , add 0.5ml water... you keep diluting it 1/2 each step.

But this is pretty basic, you should understand this, know how this works.

-pito-

Mad Researcher on Thu Nov 19 14:15:52 2015 said:

mdfenko on Thu Nov 19 14:07:30 2015 said:

take part of the 1ng/ml and add an equal volume of diluent to make 0.5ng/ml.

take part of the 0.5ng/ml and add an equal volume of diluent to make 0.25ng/ml.

take part of the 0.25ng/ml and add an equal volume of diluent to make 0.125ng/ml.

etc.

So, 1 ng/ml is my stock and if it comes in 1ml then:
I take 0.5 ml of my stock + 0.5 of H2O -- 1

0.5 ml of 1 + 0.5 ml of H2O -- 2

0.5 ml of 2 + 0.5 ml of H20 -- 3

and so on

Am i correct?

if 1, 2 and 3 are 0.5ng/ml, 0.25ng/ml and 0.125ng/ml, respectively, then yes.

-mdfenko-