Good parafilm - (Aug/04/2015 )

Hello all,

I am looking for a good brand of parafilm.

At the moment I am using parafilm from "parafilm" itself (parafilm M), but this one starts to melt even at 37°C after 1 day of incubation.

Not acceptable for me.

So any other brands you are familiar with?

Other also had this problem of melting parafilm at even low (37°C) temperatures?

Probably not going to solve your problem, but I use plastic bags instead of parafilm on my plates. Easy, quick, and they don't melt.

I was thinking the same, but wonder if it would really help.

I have to incubate over a period of 10-15 days at 37°C and using the bag alone, I wonder if this would do the trick.

You close the bag by taping it shut or?

pito on Tue Aug 4 19:36:40 2015 said:

You close the bag by taping it shut or?

I use a heat sealer to shut the bag , creates a perfect seal. Another good option is to use zip-lock bags or get some bag snap seals (don't know the proper term, but basically a crocodile clip that can be used to close the bag).

Usually Parafilm should melt at 60°C, therefore I'd complain.
Nescofilm was an alternative, but seems to be cancelled (Fisher Scientific).

hobglobin on Tue Aug 4 21:02:28 2015 said:

Usually Parafilm should melt at 60°C, therefore I'd complain.
Nescofilm was an alternative, but seems to be cancelled (Fisher Scientific).

I know!

I complained, but complaining is not solving my problem haha.

bob1 on Tue Aug 4 20:27:51 2015 said:

 

pito on Tue Aug 4 19:36:40 2015 said:

You close the bag by taping it shut or?

I use a heat sealer to shut the bag , creates a perfect seal. Another good option is to use zip-lock bags or get some bag snap seals (don't know the proper term, but basically a crocodile clip that can be used to close the bag).

 

I was thinking something similar, but I wonder if this would do the trick for an incubation period of 10-15 days....

Not really sure for what you use it, needs it to be permeable to air? I guess then you have no or very few alternatives...

Aluminium or plastic wrap might be alternatives too, though you cannot look them completely.

If you need a bag for incubation of Petri dish piles, we use the original plastic bag and seal it with a locking clip or a peg.

The major problem at 10-15 days in a bag tends to be mold growth. But you may be more sterile than I am.

hobglobin on Wed Aug 5 17:38:29 2015 said:

Not really sure for what you use it, needs it to be permeable to air? I guess then you have no or very few alternatives...

Aluminium or plastic wrap might be alternatives too, though you cannot look them completely.

If you need a bag for incubation of Petri dish piles, we use the original plastic bag and seal it with a locking clip or a peg.

well, I am doing some yeast two hybrid screenings.

So permeable to air would be something I want/need.