Concentration question - (Jun/08/2015 )

Hello everyone,

I need help about something really simple but it makes me wonder (for some reason

Ok, I am doing binding assay of protein and RNA, I use ATP in the mixture (25mM). Usually I use 2.5 ul of that ATP in mixture which is together with buffer total 40 ul (200ng/ul of RNA and protein each beginning concentration).

Now, my question is: if I use higher concentration of RNA and protein (for example total 10 ug) and total volume is 100 ul, how much of 25 mM ATP do I need in this mixture?

Thanks for the help!!

-itmaybe-

if you are using the same final concentration of atp then just multiply by the volume increase (100/40=2.5x)

-mdfenko-

Thank you very much for your reply.

I was just wondering does higher concentration of RNA and protein require more ATP...

Ok, thanks!!

-itmaybe-