Yeast strains - description of mutations :) - (Feb/06/2015 )

Hello there!

I'm doing some homework about 6 yeast strains, that we're supposed to work with on my university in a couple of weeks.

I'd like to be well prepared, but i haven't been able to figure out what the different mutations in these strains mean for their abilities to grow on different mediums etc..

Here they are:

1. MATa ade2-n(deltaNdel) LYS2 leu2-3,112 trp1-1 his3::natMX4 met22:KIURA3

2. MATalpha ade2-1 leu2,3-112 ura3-1 trp1-1 LYS2 srs2::HIS3 rad52-R70A

3. MATalpha ade2-1 his3-11,15 leu2-? LYS2 ura3-1 trp1-1 rad52-C180A srs2::HIS3

4. MATα ade2-ISceI lys2::GAL-ISceI trp1-1 his3::hphMX4 RAD5

5. MATa ade2-1 his3-11,15 leu2-3,112 lys2Δ TRP1 rad52-R70A srs2::HIS3

6. MATa ade2-1 his3-11,15 leu2-3,112 LYS trp1-1 rad52-C180A srs2::HIS3

I know that MATa and MATalpha are the type of mating cell. Also, i know that rad52-R70A and rad52-C180A are mutations in the Rad52 gene, meaning that some function of Rad52 will be affected.

However; the rest of the nomenclature confuses me and i'm not sure what it means. Could someone please clarify? :-)

Thanks a lot!

-freud-

freud on Fri Feb 6 16:18:58 2015 said:

Hello there!

I'm doing some homework about 6 yeast strains, that we're supposed to work with on my university in a couple of weeks.

I'd like to be well prepared, but i haven't been able to figure out what the different mutations in these strains mean for their abilities to grow on different mediums etc..

Here they are:

1. MATa ade2-n(deltaNdel) LYS2 leu2-3,112 trp1-1 his3::natMX4 met22:KIURA3

2. MATalpha ade2-1 leu2,3-112 ura3-1 trp1-1 LYS2 srs2::HIS3 rad52-R70A

3. MATalpha ade2-1 his3-11,15 leu2-? LYS2 ura3-1 trp1-1 rad52-C180A srs2::HIS3

4. MATα ade2-ISceI lys2::GAL-ISceI trp1-1 his3::hphMX4 RAD5

5. MATa ade2-1 his3-11,15 leu2-3,112 lys2Δ TRP1 rad52-R70A srs2::HIS3

6. MATa ade2-1 his3-11,15 leu2-3,112 LYS trp1-1 rad52-C180A srs2::HIS3

I know that MATa and MATalpha are the type of mating cell. Also, i know that rad52-R70A and rad52-C180A are mutations in the Rad52 gene, meaning that some function of Rad52 will be affected.

However; the rest of the nomenclature confuses me and i'm not sure what it means. Could someone please clarify? :-)

Thanks a lot!

What is it exactly that you need to know?

For example: in some strains you can already see that the his3 gene has been mutated by putting another gene in it.. so the strain is auxotrophic for histidine...

-pito-

pito on Fri Feb 20 21:54:58 2015 said:

freud on Fri Feb 6 16:18:58 2015 said:

!

What is it exactly that you need to know?

For example: in some strains you can already see that the his3 gene has been mutated by putting another gene in it.. so the strain is auxotrophic for histidine...

Sorry for not responding - i had given up on the thread!

What i need is basically an idea of what the different "signs/nomenclature" mean.
Like, :: and : and some kind of overview on how mutations are generally written down - general rules/guidelines/something. It's like it's written in a language that i don't understand.

Examples:

It's difficult to tell (for me) whether or not the mutation "srs2::HIS3" means that the srs2-gene has ben inserted into HIS3, or that the HIS3 gene has been inserted into the srs2 gene for example.
I know Delta means the gene has been deleted - so i'm guessing "ade2-n(deltaNdel)" means a deletion of the ade2-n gene, but i'm not sure.
LYS2 means... what? A mutation in the LYS-biopathway, or is it just to signify WT LYS? Would LYS2 make a strain auxotrophic for lysine?

Why sometimes the capital letters and sometimes not?
Like "LYS2" vs. "lys2::GAL-IsceI" - both include lys2, but differ in the way it's written. And i'm not sure what the "lys2::GAL-IsceI" even means, too. :D

Anyhow, it's all just terribly confusing to me

Thanks in advance for your help.

-freud-

freud on Tue Feb 24 15:05:47 2015 said:

pito on Fri Feb 20 21:54:58 2015 said:

freud on Fri Feb 6 16:18:58 2015 said:

!

What is it exactly that you need to know?

For example: in some strains you can already see that the his3 gene has been mutated by putting another gene in it.. so the strain is auxotrophic for histidine...

Sorry for not responding - i had given up on the thread!

What i need is basically an idea of what the different "signs/nomenclature" mean.
Like, :: and : and some kind of overview on how mutations are generally written down - general rules/guidelines/something. It's like it's written in a language that i don't understand.

Examples:

It's difficult to tell (for me) whether or not the mutation "srs2::HIS3" means that the srs2-gene has ben inserted into HIS3, or that the HIS3 gene has been inserted into the srs2 gene for example.
I know Delta means the gene has been deleted - so i'm guessing "ade2-n(deltaNdel)" means a deletion of the ade2-n gene, but i'm not sure.
LYS2 means... what? A mutation in the LYS-biopathway, or is it just to signify WT LYS? Would LYS2 make a strain auxotrophic for lysine?

Why sometimes the capital letters and sometimes not?
Like "LYS2" vs. "lys2::GAL-IsceI" - both include lys2, but differ in the way it's written. And i'm not sure what the "lys2::GAL-IsceI" even means, too.

Anyhow, it's all just terribly confusing to me

Thanks in advance for your help.