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Daily stimulation with cytokines - (Nov/24/2014 )

I was wondering how you can make daily stimulation of monolayer culture with a cytokine?

I keep reading it in papers but they write it as "the cytokine is added daily at # ng/ml" , so I was wondering how they do it.

-H. Aly-

Make a solution, add once each day after removing old medium.

-bob1-