HELP. Methanol fixation then store in??? - (Nov/06/2014 )

Hi everyone,

I am currently co-culturing cells on glass cover slips placed in 24-well plates. They were incubated with BrdU 24h before they were fixed in 100% methanol.

After fixation, I was told to let the methanol dry and then place everything in -20C until I was ready to stain (and that's what I did). However, online, I read that the cells must be placed in 4C in PBS so that it doesn't dry.

Could someone explain this discrepancy?

Thanks!

The methanol will dehydrate the cells anyway, they can be then re-hydrated by adding PBS.  You can store them in PBS for a week or so, but not much longer.