Dilute to a working concentration - (Oct/06/2014 )

Hi,

not sure of it is the right place but..

For some reason I just cannot wrap my head around it.

I've calculated that I need 0,15 ul of a reagent to be added in 150 ul of DMEM to get a c2=0,1 ug/ml.

(0,1 ug/ml x 1,5 ml) / 1000 ug/ml = 0,00015 ml = 0,15 ul.

c2                 v2           c1                   v1

However, I want to dilute this to a working concentration, e.g. 1:100 to get v1 to 15 ul.

How to practically do this?

And will it not affect the end concentration (c2)?

Thanks

dendrite on Mon Oct 6 10:22:37 2014 said:

Hi,

 

not sure of it is the right place but..

 

For some reason I just cannot wrap my head around it.

 

I've calculated that I need 0,15 ul of a reagent to be added in 150 ul of DMEM to get a c2=0,1 ug/ml.

 

(0,1 ug/ml x 1,5 ml) / 1000 ug/ml = 0,00015 ml = 0,15 ul.

c2                 v2           c1                   v1

 

 

 

However, I want to dilute this to a working concentration, e.g. 1:100 to get v1 to 15 ul.

 

 

How to practically do this?

 

And will it not affect the end concentration (c2)?

 

 

Thanks

 

 

add 1 ul of the stock to 99 ul of diluent.

the only variable affected by changing c1 is v1, which is what you wanted.