Obtaining Serum from frozen EDTA blood or cleaning reddish serum? - (Jun/14/2014 )
Hi, I have two tubes from the same patient; one is gel-serum tube (yellow cap), the other is EDTA whole blood (mauve cap), when I centrifuged the gel-serum tube the serum came out bloody (reddish). I need the serum for Vitamin analysis, can I obtain another serum from the frozen whole blood tube or is there is a way to clean the serum sample?
P.S. The color of the resulting serum is between the two colors in the image attached
how are you performing your vitamin analysis?
when i did it, i precipitated the proteins (it was a long time ago so i'm not sure, but i think i used acetonitrile to precipitate). that eliminated the hemoglobin from the serum.