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Bacterial Growth Question - (May/01/2014 )

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ah I see! When conducting the experiment I did find that when both strains were exposed to UV for 5+ mins there was no growth at all. However, in the case of the k12, exposure for 5 seconds showed only 2 individual colonies, where as with the strain B, even at 2 minutes exposure there was still around 80~ odd colonies. So, I mean, from that it does show that the k12 is more sensitive to UV than the strain B. Or maybe I should say, more sensitive at those particular wavelengths.

But thank you very much for your help, it is very much appreciated.

-Brand New-

You should specify the type of UV used (A, B, C) and if possible the wavelength range.

-bob1-

It's UVC, however, with the fish tank I couldn't find out which particular wavelength it emits, so I don't even know if it may be more than one wavelength. During my 6 weeks though I did use a UV spectrophotometer and I was getting results showing a wavelength of 251nm was the optimum and I repeated my experiment and still found it was the best, but when I looked it up, other people were saying it was around 260nm so who knows. Maybe it was an anomaly or I messed up the experiment twice! 

-Brand New-

Most UVC lamps emit at 254 nm nominally. I don't know how sharp that peak is, but likely quite sharp, since it is a mercury emission line.

-phage434-

Oh really? thank you, that's helps a lot to know that since that is close to the 251nm which I was using on the spec. and it will give a good comparison.

-Brand New-
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