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Methanol/acetic acid fixation - (Apr/22/2014 )

Dear Forum members,


I have a basic question but I would like to know if there is any reasons why the metanol/acetic acid mix used for fixation has to be prepared at the moment of use. Isn´t it possible to keep a stock stored at -20ºC for example? Also, I am interested in fixing cells for qFISH. Is this the best choice to fix cells for telomere visualization?

Thanks a lot in advance!



It is possible that there could be some esterification going on (usually requires sulphuric acid though).


No idea about the telomeres.


more likely to be a problem with evaporation.