HPLC detection of 5-methylcytidine - (Apr/12/2014 )
I have DNA of reducing folate concentration digested with Nuclease P1 hoping to show the differences in 5-methylcytidine for my undergrad senior seminar class. However, I cannot find a place that sells the standard 5-methylcytidine, and therefore, I have no idea which peak it is or if it is even showing. Any ideas on how I can quantify it?
Many people sell it. Sigma, for instance:
My bad, I meant 5-methyl-2'deoxycytosine
Many people sell it: