how to diminish the background in immunocytochemistry of yh2ax - (Apr/12/2014 )

Hello

I would like to know if someone can tell me how to diminish the background in immunocytochemistry of yh2ax

and which method is better to quantify it.

Thanks.

tell us what you have done thus far.

usually high background is due to poor blocking and/or (lack of) specificity of an antibody.