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Colony screening - (Apr/06/2014 )

I always did a RE on colony after mini-prep to check my insert. However, I always have the issue that my insert is always darker and intense than my vector after RE. and the size is always around 3kb. Any idea how does that happen? and after try another RE, it's the same, apparently it's not the right thing I want.

-weilun-

Can you post a picture?

-Trof-