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questions on transwell assay - (Mar/06/2014 )

Hi, I am facing the problems when I do transwell assay to detect cell migration. But the cells looks very "ugly" when i take pictures. Does anyone know how to keep the cell pretty? I wash the transwell with PBS, did I wash it too much? 

-Evaaaa-

Can you add a pic of these "ugly" cells?  At which point are they "ugly" - before or after migration?

-bob1-

Can you add a pic of these "ugly" cells?  At which point are they "ugly" - before or after migration?

After migration. They look "broken" 

-Evaaaa-

What sort of broken?  apoptotic? senescent? ragged? vacuolated? change of shape?

 

What cells are these?  Is is possible that they are differentiating?

-bob1-