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Insect histology - (Jan/14/2014 )



i trying to include triatomine antena in metachrylate, but the results is no good. Someone have any sugestion to fixation and inclusion to chitin rich tissue?




One way might be to soften or even remove the chitin (dechitinising). There are protocols available to do this. One way would be using concentrated sulphuric and heating it up. Or using an enzyme (chitinase)...


Btw. you posted this before in another section, better ask a moderator to change the section and then bump the topic to be visible again in the new posts list. And please don't use the "Report" button but the "Post" button when you post something.