Dot blot anomaly - (Dec/22/2013 )
I carried out a dot blot for various concentrations of my cell lysate and found weak positive staining for my protein of interest at a concentration of 15-25ng. However there was absolutely no staining at higher lysate concentrations (the positive control stained even at 100ng). Can someone tell me why? Thanks!
was the positive control a lysate or purified protein?
The positive control was also a whole cell lysate