Protocol Online logo
Top : New Forum Archives (2009-): : General Lab Techniques

How to measure lactic acid concentration? - (Oct/13/2013 )

Hi,

 

I am unsure of whether this is the correct section to ask this question, but, does anyone know how to measure the concentration of lactic acid? Not in blood/tissues. The lactic acid is embedded in some sort of absorptive matrix. Our lab received this for analysis but no one knows exactly how to measure it.

-hianghao-

can you extract the lactic acid from absorptive matrix?

 

(homogenize in some medium, centrifuge to remove insoluble material)

-mdfenko-

can you extract the lactic acid from absorptive matrix?

 

(homogenize in some medium, centrifuge to remove insoluble material)

What kind of medium would be suitable? Any solvent?

-hianghao-

can you try out an enzymatic assay and then spectrophotometric measurement. It should work if the enzyme is not inhibited by the absorption of the lactic acid to the matrix. Then you don't have to extract it (which has to be quantitative).

-hobglobin-

can you try out an enzymatic assay and then spectrophotometric measurement. It should work if the enzyme is not inhibited by the absorption of the lactic acid to the matrix. Then you don't have to extract it (which has to be quantitative).

Thanks! I'll try this!

-hianghao-

 

can you extract the lactic acid from absorptive matrix?

 

(homogenize in some medium, centrifuge to remove insoluble material)

What kind of medium would be suitable? Any solvent?

 

if you decide to try this then you can use any medium compatible with the assay,

-mdfenko-